Novel enriched pathways in superficial malignant peripheral nerve sheath tumours and spindle/desmoplastic melanomas.
Identifieur interne : 000537 ( Main/Exploration ); précédent : 000536; suivant : 000538Novel enriched pathways in superficial malignant peripheral nerve sheath tumours and spindle/desmoplastic melanomas.
Auteurs : George Jour [États-Unis] ; Nicole K. Andeen [États-Unis] ; Rami Al-Rohil [États-Unis] ; Phyu P. Aung [États-Unis] ; Meenakshi Mehrotra [États-Unis] ; Dzifa Duose [États-Unis] ; Benjamin Hoch [États-Unis] ; Zolt Argenyi [États-Unis] ; Rajyalakshmi Luthra [États-Unis] ; Ignacio I. Wistuba [États-Unis] ; Victor G. Prieto [États-Unis]Source :
- The Journal of pathology [ 1096-9896 ] ; 2018.
Descripteurs français
- KwdFr :
- Adulte (MeSH), Adulte d'âge moyen (MeSH), Analyse de profil d'expression de gènes (MeSH), Analyse de séquence d'ADN (MeSH), Analyse de séquence d'ARN (MeSH), Banque de gènes (MeSH), Femelle (MeSH), Humains (MeSH), Microenvironnement tumoral (MeSH), Mutation (MeSH), Mâle (MeSH), Mélanome (anatomopathologie), Neurinome (anatomopathologie), Sujet âgé (MeSH), Sujet âgé de 80 ans ou plus (MeSH), Séquençage nucléotidique à haut débit (MeSH), Variations de nombre de copies de segment d'ADN (MeSH).
- MESH :
- anatomopathologie : Mélanome, Neurinome.
- Adulte, Adulte d'âge moyen, Analyse de profil d'expression de gènes, Analyse de séquence d'ADN, Analyse de séquence d'ARN, Banque de gènes, Femelle, Humains, Microenvironnement tumoral, Mutation, Mâle, Sujet âgé, Sujet âgé de 80 ans ou plus, Séquençage nucléotidique à haut débit, Variations de nombre de copies de segment d'ADN.
English descriptors
- KwdEn :
- Adult (MeSH), Aged (MeSH), Aged, 80 and over (MeSH), DNA Copy Number Variations (MeSH), Female (MeSH), Gene Expression Profiling (MeSH), Gene Library (MeSH), High-Throughput Nucleotide Sequencing (MeSH), Humans (MeSH), Male (MeSH), Melanoma (pathology), Middle Aged (MeSH), Mutation (MeSH), Neurilemmoma (pathology), Sequence Analysis, DNA (MeSH), Sequence Analysis, RNA (MeSH), Tumor Microenvironment (MeSH).
- MESH :
Abstract
Superficial malignant peripheral nerve sheath tumour (MPNST) is a rare, soft tissue neoplasm that shares morphological features and some molecular events with spindle/desmoplastic melanoma (SDM). Herein, we sought to identify molecular targets for therapy by using targeted RNA/DNA sequencing and gene expression of key immunological players. DNA and RNA from formalin-fixed paraffin-embedded tissue were extracted and processed. Massive high-throughput deep parallel sequencing was performed with the Oncomine comprehensive panel, enabling detection of relevant single-nucleotide variants, copy number variations, gene fusions and indels for 143 unique genes on the Ion torrent sequencer for clinical trial research programmes. Gene expression analysis was carried out with a customized 770-gene expression panel combining markers for 24 different immune cell types and 30 common cancer antigens, including key checkpoint blockade genes analysed with the Ncounter system. Fifty-one patients (SDM, 16/11; MPNST, 24; male, n = 37; female, n = 16) had sufficient DNA and RNA for testing. NF1 deleterious mutations and/or deep/homozygous deletions were identified in 73% of MPNSTs and 67% of SDMs, with 50% of the mutations involving the RAS-binding domain. Inactivating/deleterious mutations of TSC1/TSC2 were identified in 40% and 41% of MPNSTs and SDMs, respectively. Activating mutations affecting the EGFR-like and the negative regulatory domains of NOTCH1 and KDR (VEGFR2) were identified in 45% and 40% of SDMs and in 30% and 8% of MPNSTs, respectively. Differential gene expression and gene clustering analysis showed significantly perturbed immune pathway components, including nuclear factor-κB (NF-κB), JAK-STAT, and CXCL12-CXCR4, and differentially expressed CD274 and CTLA4, in both SDM and MPNST. Angiogenesis (KDR and NOTCH1) and mammalian target of rapamycin complex (mTORC) pathways offer a rationale for anti-angiogenic and selective mTORC inhibition as treatment strategies for MPNST and SDM. Cytokines and the JAK-STAT, TNF and NF-κB axes were perturbed in both SDM and MPNST. These pathways have been targeted in haematological malignancies and present promising targets for these tumours. Copyright © 2017 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
DOI: 10.1002/path.4996
PubMed: 28991373
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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<author><name sortKey="Luthra, Rajyalakshmi" sort="Luthra, Rajyalakshmi" uniqKey="Luthra R" first="Rajyalakshmi" last="Luthra">Rajyalakshmi Luthra</name>
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<author><name sortKey="Prieto, Victor G" sort="Prieto, Victor G" uniqKey="Prieto V" first="Victor G" last="Prieto">Victor G. Prieto</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult (MeSH)</term>
<term>Aged (MeSH)</term>
<term>Aged, 80 and over (MeSH)</term>
<term>DNA Copy Number Variations (MeSH)</term>
<term>Female (MeSH)</term>
<term>Gene Expression Profiling (MeSH)</term>
<term>Gene Library (MeSH)</term>
<term>High-Throughput Nucleotide Sequencing (MeSH)</term>
<term>Humans (MeSH)</term>
<term>Male (MeSH)</term>
<term>Melanoma (pathology)</term>
<term>Middle Aged (MeSH)</term>
<term>Mutation (MeSH)</term>
<term>Neurilemmoma (pathology)</term>
<term>Sequence Analysis, DNA (MeSH)</term>
<term>Sequence Analysis, RNA (MeSH)</term>
<term>Tumor Microenvironment (MeSH)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Adulte (MeSH)</term>
<term>Adulte d'âge moyen (MeSH)</term>
<term>Analyse de profil d'expression de gènes (MeSH)</term>
<term>Analyse de séquence d'ADN (MeSH)</term>
<term>Analyse de séquence d'ARN (MeSH)</term>
<term>Banque de gènes (MeSH)</term>
<term>Femelle (MeSH)</term>
<term>Humains (MeSH)</term>
<term>Microenvironnement tumoral (MeSH)</term>
<term>Mutation (MeSH)</term>
<term>Mâle (MeSH)</term>
<term>Mélanome (anatomopathologie)</term>
<term>Neurinome (anatomopathologie)</term>
<term>Sujet âgé (MeSH)</term>
<term>Sujet âgé de 80 ans ou plus (MeSH)</term>
<term>Séquençage nucléotidique à haut débit (MeSH)</term>
<term>Variations de nombre de copies de segment d'ADN (MeSH)</term>
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<keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Mélanome</term>
<term>Neurinome</term>
</keywords>
<keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Melanoma</term>
<term>Neurilemmoma</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Adult</term>
<term>Aged</term>
<term>Aged, 80 and over</term>
<term>DNA Copy Number Variations</term>
<term>Female</term>
<term>Gene Expression Profiling</term>
<term>Gene Library</term>
<term>High-Throughput Nucleotide Sequencing</term>
<term>Humans</term>
<term>Male</term>
<term>Middle Aged</term>
<term>Mutation</term>
<term>Sequence Analysis, DNA</term>
<term>Sequence Analysis, RNA</term>
<term>Tumor Microenvironment</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Adulte</term>
<term>Adulte d'âge moyen</term>
<term>Analyse de profil d'expression de gènes</term>
<term>Analyse de séquence d'ADN</term>
<term>Analyse de séquence d'ARN</term>
<term>Banque de gènes</term>
<term>Femelle</term>
<term>Humains</term>
<term>Microenvironnement tumoral</term>
<term>Mutation</term>
<term>Mâle</term>
<term>Sujet âgé</term>
<term>Sujet âgé de 80 ans ou plus</term>
<term>Séquençage nucléotidique à haut débit</term>
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<front><div type="abstract" xml:lang="en">Superficial malignant peripheral nerve sheath tumour (MPNST) is a rare, soft tissue neoplasm that shares morphological features and some molecular events with spindle/desmoplastic melanoma (SDM). Herein, we sought to identify molecular targets for therapy by using targeted RNA/DNA sequencing and gene expression of key immunological players. DNA and RNA from formalin-fixed paraffin-embedded tissue were extracted and processed. Massive high-throughput deep parallel sequencing was performed with the Oncomine comprehensive panel, enabling detection of relevant single-nucleotide variants, copy number variations, gene fusions and indels for 143 unique genes on the Ion torrent sequencer for clinical trial research programmes. Gene expression analysis was carried out with a customized 770-gene expression panel combining markers for 24 different immune cell types and 30 common cancer antigens, including key checkpoint blockade genes analysed with the Ncounter system. Fifty-one patients (SDM, 16/11; MPNST, 24; male, n = 37; female, n = 16) had sufficient DNA and RNA for testing. NF1 deleterious mutations and/or deep/homozygous deletions were identified in 73% of MPNSTs and 67% of SDMs, with 50% of the mutations involving the RAS-binding domain. Inactivating/deleterious mutations of TSC1/TSC2 were identified in 40% and 41% of MPNSTs and SDMs, respectively. Activating mutations affecting the EGFR-like and the negative regulatory domains of NOTCH1 and KDR (VEGFR2) were identified in 45% and 40% of SDMs and in 30% and 8% of MPNSTs, respectively. Differential gene expression and gene clustering analysis showed significantly perturbed immune pathway components, including nuclear factor-κB (NF-κB), JAK-STAT, and CXCL12-CXCR4, and differentially expressed CD274 and CTLA4, in both SDM and MPNST. Angiogenesis (KDR and NOTCH1) and mammalian target of rapamycin complex (mTORC) pathways offer a rationale for anti-angiogenic and selective mTORC inhibition as treatment strategies for MPNST and SDM. Cytokines and the JAK-STAT, TNF and NF-κB axes were perturbed in both SDM and MPNST. These pathways have been targeted in haematological malignancies and present promising targets for these tumours. Copyright © 2017 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.</div>
</front>
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</Journal>
<ArticleTitle>Novel enriched pathways in superficial malignant peripheral nerve sheath tumours and spindle/desmoplastic melanomas.</ArticleTitle>
<Pagination><MedlinePgn>97-106</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1002/path.4996</ELocationID>
<Abstract><AbstractText>Superficial malignant peripheral nerve sheath tumour (MPNST) is a rare, soft tissue neoplasm that shares morphological features and some molecular events with spindle/desmoplastic melanoma (SDM). Herein, we sought to identify molecular targets for therapy by using targeted RNA/DNA sequencing and gene expression of key immunological players. DNA and RNA from formalin-fixed paraffin-embedded tissue were extracted and processed. Massive high-throughput deep parallel sequencing was performed with the Oncomine comprehensive panel, enabling detection of relevant single-nucleotide variants, copy number variations, gene fusions and indels for 143 unique genes on the Ion torrent sequencer for clinical trial research programmes. Gene expression analysis was carried out with a customized 770-gene expression panel combining markers for 24 different immune cell types and 30 common cancer antigens, including key checkpoint blockade genes analysed with the Ncounter system. Fifty-one patients (SDM, 16/11; MPNST, 24; male, n = 37; female, n = 16) had sufficient DNA and RNA for testing. NF1 deleterious mutations and/or deep/homozygous deletions were identified in 73% of MPNSTs and 67% of SDMs, with 50% of the mutations involving the RAS-binding domain. Inactivating/deleterious mutations of TSC1/TSC2 were identified in 40% and 41% of MPNSTs and SDMs, respectively. Activating mutations affecting the EGFR-like and the negative regulatory domains of NOTCH1 and KDR (VEGFR2) were identified in 45% and 40% of SDMs and in 30% and 8% of MPNSTs, respectively. Differential gene expression and gene clustering analysis showed significantly perturbed immune pathway components, including nuclear factor-κB (NF-κB), JAK-STAT, and CXCL12-CXCR4, and differentially expressed CD274 and CTLA4, in both SDM and MPNST. Angiogenesis (KDR and NOTCH1) and mammalian target of rapamycin complex (mTORC) pathways offer a rationale for anti-angiogenic and selective mTORC inhibition as treatment strategies for MPNST and SDM. Cytokines and the JAK-STAT, TNF and NF-κB axes were perturbed in both SDM and MPNST. These pathways have been targeted in haematological malignancies and present promising targets for these tumours. Copyright © 2017 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.</AbstractText>
<CopyrightInformation>Copyright © 2017 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Jour</LastName>
<ForeName>George</ForeName>
<Initials>G</Initials>
<Identifier Source="ORCID">0000-0001-8916-8966</Identifier>
<AffiliationInfo><Affiliation>Department of Pathology and Laboratory Medicine, MD Anderson Cancer Center at Cooper, One Cooper Plaza, Camden, NJ, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Andeen</LastName>
<ForeName>Nicole K</ForeName>
<Initials>NK</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, University of Washington School of Medicine, 1959 NE Pacific Street, Seattle, WA, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Al-Rohil</LastName>
<ForeName>Rami</ForeName>
<Initials>R</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Aung</LastName>
<ForeName>Phyu P</ForeName>
<Initials>PP</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, Section of Dermatopathology, the University of Texas - MD Anderson Cancer Center, Houston, TX, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Mehrotra</LastName>
<ForeName>Meenakshi</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Department of Translational Molecular Pathology, The University of Texas - MD Anderson Cancer Center, Houston, TX, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Duose</LastName>
<ForeName>Dzifa</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>Department of Translational Molecular Pathology, The University of Texas - MD Anderson Cancer Center, Houston, TX, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hoch</LastName>
<ForeName>Benjamin</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, University of Washington School of Medicine, 1959 NE Pacific Street, Seattle, WA, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Argenyi</LastName>
<ForeName>Zolt</ForeName>
<Initials>Z</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, University of Washington School of Medicine, 1959 NE Pacific Street, Seattle, WA, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Luthra</LastName>
<ForeName>Rajyalakshmi</ForeName>
<Initials>R</Initials>
<AffiliationInfo><Affiliation>Department of Hematopathology, The University of Texas - MD Anderson Cancer Center, Houston, TX, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wistuba</LastName>
<ForeName>Ignacio I</ForeName>
<Initials>II</Initials>
<AffiliationInfo><Affiliation>Department of Translational Molecular Pathology, The University of Texas - MD Anderson Cancer Center, Houston, TX, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Prieto</LastName>
<ForeName>Victor G</ForeName>
<Initials>VG</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, Section of Dermatopathology, the University of Texas - MD Anderson Cancer Center, Houston, TX, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Translational Molecular Pathology, The University of Texas - MD Anderson Cancer Center, Houston, TX, USA.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>J Pathol</MedlineTA>
<NlmUniqueID>0204634</NlmUniqueID>
<ISSNLinking>0022-3417</ISSNLinking>
</MedlineJournalInfo>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000368" MajorTopicYN="N">Aged</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000369" MajorTopicYN="N">Aged, 80 and over</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D056915" MajorTopicYN="N">DNA Copy Number Variations</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015723" MajorTopicYN="N">Gene Library</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D059014" MajorTopicYN="N">High-Throughput Nucleotide Sequencing</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008545" MajorTopicYN="N">Melanoma</DescriptorName>
<QualifierName UI="Q000473" MajorTopicYN="Y">pathology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009442" MajorTopicYN="N">Neurilemmoma</DescriptorName>
<QualifierName UI="Q000473" MajorTopicYN="Y">pathology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017423" MajorTopicYN="N">Sequence Analysis, RNA</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D059016" MajorTopicYN="N">Tumor Microenvironment</DescriptorName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">gene expression</Keyword>
<Keyword MajorTopicYN="Y">immune blockade</Keyword>
<Keyword MajorTopicYN="Y">spindle cell melanoma, desmoplastic melanoma</Keyword>
<Keyword MajorTopicYN="Y">superficial malignant peripheral nerve sheath tumour</Keyword>
<Keyword MajorTopicYN="Y">targeted RNA/DNA sequencing</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year>
<Month>06</Month>
<Day>07</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2017</Year>
<Month>10</Month>
<Day>03</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2017</Year>
<Month>10</Month>
<Day>04</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2017</Year>
<Month>10</Month>
<Day>11</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2018</Year>
<Month>1</Month>
<Day>10</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2017</Year>
<Month>10</Month>
<Day>10</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">28991373</ArticleId>
<ArticleId IdType="doi">10.1002/path.4996</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>New Jersey</li>
<li>Tennessee</li>
<li>Texas</li>
<li>Washington (État)</li>
</region>
</list>
<tree><country name="États-Unis"><region name="New Jersey"><name sortKey="Jour, George" sort="Jour, George" uniqKey="Jour G" first="George" last="Jour">George Jour</name>
</region>
<name sortKey="Al Rohil, Rami" sort="Al Rohil, Rami" uniqKey="Al Rohil R" first="Rami" last="Al-Rohil">Rami Al-Rohil</name>
<name sortKey="Andeen, Nicole K" sort="Andeen, Nicole K" uniqKey="Andeen N" first="Nicole K" last="Andeen">Nicole K. Andeen</name>
<name sortKey="Argenyi, Zolt" sort="Argenyi, Zolt" uniqKey="Argenyi Z" first="Zolt" last="Argenyi">Zolt Argenyi</name>
<name sortKey="Aung, Phyu P" sort="Aung, Phyu P" uniqKey="Aung P" first="Phyu P" last="Aung">Phyu P. Aung</name>
<name sortKey="Duose, Dzifa" sort="Duose, Dzifa" uniqKey="Duose D" first="Dzifa" last="Duose">Dzifa Duose</name>
<name sortKey="Hoch, Benjamin" sort="Hoch, Benjamin" uniqKey="Hoch B" first="Benjamin" last="Hoch">Benjamin Hoch</name>
<name sortKey="Luthra, Rajyalakshmi" sort="Luthra, Rajyalakshmi" uniqKey="Luthra R" first="Rajyalakshmi" last="Luthra">Rajyalakshmi Luthra</name>
<name sortKey="Mehrotra, Meenakshi" sort="Mehrotra, Meenakshi" uniqKey="Mehrotra M" first="Meenakshi" last="Mehrotra">Meenakshi Mehrotra</name>
<name sortKey="Prieto, Victor G" sort="Prieto, Victor G" uniqKey="Prieto V" first="Victor G" last="Prieto">Victor G. Prieto</name>
<name sortKey="Prieto, Victor G" sort="Prieto, Victor G" uniqKey="Prieto V" first="Victor G" last="Prieto">Victor G. Prieto</name>
<name sortKey="Wistuba, Ignacio I" sort="Wistuba, Ignacio I" uniqKey="Wistuba I" first="Ignacio I" last="Wistuba">Ignacio I. Wistuba</name>
</country>
</tree>
</affiliations>
</record>
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